Difference between revisions of "Part:BBa K1469009"
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2018 SSTi-SZGD erified that glmU increases HA production. glmU encodes a UDP-N-acetylglucosamine pyrophosphorylase. it has 495 amino aicd (also know as : GcaD in megaterium)<br> | 2018 SSTi-SZGD erified that glmU increases HA production. glmU encodes a UDP-N-acetylglucosamine pyrophosphorylase. it has 495 amino aicd (also know as : GcaD in megaterium)<br> | ||
168E-glmU produces precipitates formed by the reaction of HA with CTAB, OD400:2.313. HA’s concentration is 447.2mg/L. | 168E-glmU produces precipitates formed by the reaction of HA with CTAB, OD400:2.313. HA’s concentration is 447.2mg/L. |
Revision as of 04:02, 14 October 2018
gcaD
UDP-N-acetylglucosamine pyrophosphorylase of Bacillus megaterium. The enzyme is bifunctional and catalyses the conversion of glucosamine 1-phosphate to N-acetyl glucosamine 1-phosphate as well as the conversion of N-acetyl glucosamine 1-phosphate to UDP-N-acetyl glucosamine, a key precursor molecule for hyaluronic acid production.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 768
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 220
=====Improvement=====
2018 SSTi-SZGD erified that glmU increases HA production. glmU encodes a UDP-N-acetylglucosamine pyrophosphorylase. it has 495 amino aicd (also know as : GcaD in megaterium)
168E-glmU produces precipitates formed by the reaction of HA with CTAB, OD400:2.313. HA’s concentration is 447.2mg/L.