Difference between revisions of "Part:BBa K2623026"
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<partinfo>BBa_K2623026 short</partinfo> | <partinfo>BBa_K2623026 short</partinfo> | ||
| − | + | ===Summary=== | |
Archaeal ribosomal protein protein L7Ae owns the ability to bind with RNA with C/Dbox RNA structure. Through the isopeptide bonds formed between SpyTag and SpyCatcher, L7Ae can be anchored with OmpA, and then secreted into OMVs. To transport our L7Ae into the periplasmic space, we insert TorA leader sequence to the circuitry. It's a non-RFP form of <partinfo>BBa_K2623025</partinfo> and will be used to construct our total circuit. | Archaeal ribosomal protein protein L7Ae owns the ability to bind with RNA with C/Dbox RNA structure. Through the isopeptide bonds formed between SpyTag and SpyCatcher, L7Ae can be anchored with OmpA, and then secreted into OMVs. To transport our L7Ae into the periplasmic space, we insert TorA leader sequence to the circuitry. It's a non-RFP form of <partinfo>BBa_K2623025</partinfo> and will be used to construct our total circuit. | ||
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| + | ===Usage and Biology=== | ||
| + | To ensure which time point we should add the inducer IPTG, we measured the growth curve of BBa_K2623026 by detecting the OD 600 nm of E. coli(BL21) every hour. | ||
| + | <br><table><tr><th> | ||
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Revision as of 12:26, 17 October 2018
Archaeal ribosomal protein protein L7Ae fused with SpyCatcher.
Summary
Archaeal ribosomal protein protein L7Ae owns the ability to bind with RNA with C/Dbox RNA structure. Through the isopeptide bonds formed between SpyTag and SpyCatcher, L7Ae can be anchored with OmpA, and then secreted into OMVs. To transport our L7Ae into the periplasmic space, we insert TorA leader sequence to the circuitry. It's a non-RFP form of BBa_K2623025 and will be used to construct our total circuit.
Usage and Biology
To ensure which time point we should add the inducer IPTG, we measured the growth curve of BBa_K2623026 by detecting the OD 600 nm of E. coli(BL21) every hour.
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Sequence and Features
Assembly Compatibility:
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