Difference between revisions of "Part:BBa K2558204:Design"
 
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===Ptac test device with Anderson medium-expressed lacI===
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__NOTOC__
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<partinfo>BBa_K2558204 short</partinfo>
 
<partinfo>BBa_K2558204 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2558204 SequenceAndFeatures</partinfo>
  
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===Design Note===
 
===Design Note===
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In order to investigate how lacI dosage affect IPTG induction we used Anderson promotor J23100, J23110 and J23114 to design three constitutive lacI generator of different intensity. The three lacI generator were then ligated with Ptac controlled reporter sfGFP to make three IPTG induction devices (https://parts.igem.org/Part:BBa_K2558203, https://parts.igem.org/Part:BBa_K2558204, https://parts.igem.org/Part:BBa_K2558205). By measuring sfGFP fluorescence we tested how these devices react to IPTG.
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In order to investigate how lacI dosage affects IPTG induction, we used Anderson promotor J23100, J23110 and J23114 to design three constitutive lacI generators of different intensities. The three lacI generators were then ligated with Ptac driven reporter sfGFP to make three IPTG induction devices (BBa_K2558203, BBa_K2558204, BBa_K2558205). By measuring sfGFP fluorescence we tested how these devices react to IPTG.
  
  

Latest revision as of 16:23, 17 October 2018

Ptac test device with Anderson medium-expressed lacI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1073
    Illegal NheI site found at 1096
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2277
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 176


Design Note

In order to investigate how lacI dosage affects IPTG induction, we used Anderson promotor J23100, J23110 and J23114 to design three constitutive lacI generators of different intensities. The three lacI generators were then ligated with Ptac driven reporter sfGFP to make three IPTG induction devices (BBa_K2558203, BBa_K2558204, BBa_K2558205). By measuring sfGFP fluorescence we tested how these devices react to IPTG.


Source

References