Difference between revisions of "Part:BBa K2728004"
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| − | === | + | === Sequence === |
| + | We ordered the synthesized plasmid from Genscript. After restriction digestion & transformation, we got our E. coli with GFA. | ||
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| + | [[File:T--BGIC-Global--gfasyn1.png|left|caption]]<br clear=all> | ||
| + | [[File:T--BGIC-Global--gfasyn2.png|left|caption]]<br clear=all> | ||
| + | ===== Fig 3: Order info ===== | ||
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Revision as of 03:53, 14 October 2018
GFA - A Glutathione-Dependent Formaldehyde-Activating Enzyme
Basic Description
An enzyme from Paracoccus denitrificans that accelerates this spontaneous condensation reaction, which catalyze the conversion of formaldehyde and glutathione was purified and named glutathione-dependent formaldehyde-activating enzyme (Gfa).
The gene GFA is located directly upstream of the gene for glutathione-dependent formaldehyde dehydrogenase, which catalyzes the subsequent oxidation of S-hydroxymethylglutathione. The glutathione-dependent formaldehyde conversion to formate starts with the adduct formation, formaldehyde reacts with the SH group of glutathione producing S-hydroxymethylglutathione (Reaction 1).
Fig 1: Thiol-dependent pathway
Formaldehyde-converting enzymes-Gfa is composed of one type of subunit of about 20 kDa and lack a chromophoric prosthetic group. In addition, both enzymes are encoded next to genes for enzymes involved in further oxidation of the cofactor-bound one-carbon unit to carbon dioxide.
Fig 2: 3D structure (from www.uniprot.org)
Sequence
We ordered the synthesized plasmid from Genscript. After restriction digestion & transformation, we got our E. coli with GFA.
Fig 3: Order info
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]