Difference between revisions of "Part:BBa K091104:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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| + | <partinfo>BBa_K091104 AddReview number</partinfo> | ||
| + | <I>SarahV</I> | ||
| + | |width='60%' valign='top'| | ||
| + | We transformed Bba_K091104 (=pLac/Mnt hybrid) in DH5α-cells, followed by a minipreparation. After this we did a restriction digest with EcoRI and SpeI. Since the pSB1A2 vector is 2079bp and the Lac/Mnt hybrid promoter should be 87bp, we also expect a fragment of 87bp, one of 2079 and one of 2166bp. | ||
| + | However, we see a fragment of 1000bp and of ±2000bp. | ||
| + | |} | ||
| + | [[Image:Restriction_1807.jpg]] | ||
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Revision as of 11:23, 9 August 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K091104
User Reviews
UNIQf6890e866ff7896e-partinfo-00000000-QINU
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No review score entered. SarahV |
We transformed Bba_K091104 (=pLac/Mnt hybrid) in DH5α-cells, followed by a minipreparation. After this we did a restriction digest with EcoRI and SpeI. Since the pSB1A2 vector is 2079bp and the Lac/Mnt hybrid promoter should be 87bp, we also expect a fragment of 87bp, one of 2079 and one of 2166bp. However, we see a fragment of 1000bp and of ±2000bp. |
UNIQf6890e866ff7896e-partinfo-00000002-QINU