Difference between revisions of "Part:BBa K2680115"
 
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<partinfo>BBa_K2680115 short</partinfo>
 
<partinfo>BBa_K2680115 short</partinfo>
  
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3G-compatible version of Lux-activated promoter <partinfo>BBa_R0063</partinfo>. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].  
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[https://parts.igem.org/Part:BBa_R0063 Lux-activated promoter pLuxR-pR] flanked by prefix sticky end A and suffix sticky end B in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].  
  
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Flanked by prefix sticky end A and suffix sticky end B.
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From the registry parts page for pLuxR-pR: "The lux cassette of V. fischeri contains a left and a right promoter. The left promoter gives weak constitutive expression of downstream genes.This expression is down-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription from Pr, repressing transcription from Pl."<sup>1</sup>
  
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===Usage and Biology===
 
===Usage and Biology===
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"Medium strength promoter in the absence of LuxR/HSL."<sup>1</sup>
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===References===
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<sup>1</sup>Mahajan, V. S., Marinescu, V. D., Chow, B., Wissner-Gross, A. D., & Carr, P. (2003, January 31). Part:BBa_R0063. Retrieved October 14, 2018, from https://parts.igem.org/Part:BBa_R0063
  
 
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Latest revision as of 23:10, 14 October 2018


3G pLuxR-pR

Lux-activated promoter pLuxR-pR flanked by prefix sticky end A and suffix sticky end B in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

From the registry parts page for pLuxR-pR: "The lux cassette of V. fischeri contains a left and a right promoter. The left promoter gives weak constitutive expression of downstream genes.This expression is down-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription from Pr, repressing transcription from Pl."1

Usage and Biology

"Medium strength promoter in the absence of LuxR/HSL."1

References

1Mahajan, V. S., Marinescu, V. D., Chow, B., Wissner-Gross, A. D., & Carr, P. (2003, January 31). Part:BBa_R0063. Retrieved October 14, 2018, from https://parts.igem.org/Part:BBa_R0063

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 47
    Illegal BsaI.rc site found at 55
    Illegal BsaI.rc site found at 214