Difference between revisions of "Part:BBa K2841510"
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The dCAS9 protein used in the [https://www.ncbi.nlm.nih.gov/pubmed/23761437 CRISPR repressor 2013 paper]. The sequence of the part has been modified to be compatible with iGEM standard. The dCAS9 accepts a gRNA (<bbpart>BBa_K2841514</bbpart>) which directs it to a site of interest. The [https://www.rcsb.org/structure/4ZT9 structure for the protein] family has been solved. | The dCAS9 protein used in the [https://www.ncbi.nlm.nih.gov/pubmed/23761437 CRISPR repressor 2013 paper]. The sequence of the part has been modified to be compatible with iGEM standard. The dCAS9 accepts a gRNA (<bbpart>BBa_K2841514</bbpart>) which directs it to a site of interest. The [https://www.rcsb.org/structure/4ZT9 structure for the protein] family has been solved. | ||
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+ | https://static.igem.org/mediawiki/parts/6/66/T--Warwick--2018-4zt9.gif | ||
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Revision as of 18:37, 13 October 2018
Streptococcus Pyogenes dCAS9
The dCAS9 protein used in the CRISPR repressor 2013 paper. The sequence of the part has been modified to be compatible with iGEM standard. The dCAS9 accepts a gRNA (BBa_K2841514) which directs it to a site of interest. The structure for the protein family has been solved.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]