Difference between revisions of "Part:BBa K2865006"
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===Experiment=== | ===Experiment=== | ||
<B>Fluorescence microscope</B> | <B>Fluorescence microscope</B> | ||
− | <p class="MsoNormal"><span lang="EN-US">We transfected H9C2 cell with target plasmids, BBa_K2865006 and BBa_K2865005, via liposome transfection. After 48 hours’ expression, we detected the cells using fluorescence microscope. And we have taken clearly photos. | + | <p class="MsoNormal"><span lang="EN-US">We transfected H9C2 cell with target plasmids, BBa_K2865006 and BBa_K2865005, via liposome transfection. After 48 hours’ expression, we detected the cells using fluorescence microscope. And we have taken clearly photos. We all know that the CMV promoter is widely and commonly used in plasmids or viruses designed for gene expression in mammalian cells since it is a very strong promoter and drives constitutive expression of genes under its control. And we have to admit that the transfection efficiency is low. </span></p> |
<p class="MsoNormal" style="text-indent: 0cm;"><span lang="EN-US"> </span></p> | <p class="MsoNormal" style="text-indent: 0cm;"><span lang="EN-US"> </span></p> | ||
[[File:T--SMMU-China--cep.png|500px|thumb|center|Figure 2 Expression of CMV-eGFP-poly(A) was taken under fluorescent microscope and light microscope.]] | [[File:T--SMMU-China--cep.png|500px|thumb|center|Figure 2 Expression of CMV-eGFP-poly(A) was taken under fluorescent microscope and light microscope.]] |
Latest revision as of 12:01, 15 October 2018
CMV promoter-eGFP-poly(A)
This part contains CMV promoter, eGFP reporter in RFC25 format(BBa_K2865005), and SV40 polyA terminator (BBa_K2865004). This part is constructed as a control with our part BNP promoter-eGFP-poly(A) (BBa_K2865008).
Experiment
Fluorescence microscope
We transfected H9C2 cell with target plasmids, BBa_K2865006 and BBa_K2865005, via liposome transfection. After 48 hours’ expression, we detected the cells using fluorescence microscope. And we have taken clearly photos. We all know that the CMV promoter is widely and commonly used in plasmids or viruses designed for gene expression in mammalian cells since it is a very strong promoter and drives constitutive expression of genes under its control. And we have to admit that the transfection efficiency is low.
CMV promoter-eGFP-poly(A) served as the positive control. It contains the CMV promoter and eGFP reporter.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1430
- 1000COMPATIBLE WITH RFC[1000]