Difference between revisions of "Part:BBa K2753055"
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<partinfo>BBa_K2753055 short</partinfo>
 
<partinfo>BBa_K2753055 short</partinfo>
  
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The promoter UP119 is a modified variant of J23119 consensus promoter[[Parts:BBa_J23119]] by adding an up-element. This alteration makes UP119 stronger than the original J23119.
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The promoter UP119 is a modified variant of J23119 consensus promoter ([[Parts:BBa_J23119]]) by adding an up-element. This alteration makes UP119 stronger than the original J23119.
  
 
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Revision as of 15:15, 13 October 2018


UPJ23119

The promoter UP119 is a modified variant of J23119 consensus promoter (Parts:BBa_J23119) by adding an up-element. This alteration makes UP119 stronger than the original J23119.


For comparing the strength of two J23119 variants with J23119, we cloned them with a sfGFP onto three plasmids of distinctive copy numbers.

  • pUC20: ~500
  • pR6K: ~15
  • pSC101: ~1


The strength of them are characterised by measuring the fluorescence using a Flow Cytometry.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 27
    Illegal NheI site found at 50
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]