Difference between revisions of "Part:BBa K2623024"

Latest revision as of 03:52, 14 October 2018

A non-GFP form of the BBa_K2623023

This part is the non-GFP form of BBa_K2623023. It's also another form of BBa_K2623022 in which SpyTag is inserted to the C-termini of OmpA and is designed to target our archaeal ribosomal protein protein L7Ae and then siRNA into outer-membrane vesicles (OMVs). Porin outer-membrane protein A, OmpA, is a kind of highly expressed transmembrane porin protein of E. coli and is therefore abundant in OMVs. We insert SpyTag to its C-termini and SpyCather to L7Ae (BBa_K2623026) in order to anchore L7Ae to outer membrane through the bio-conjugation of the SpyTag/SpyCatcher system.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 65
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2623024 short</partinfo>
-This part was the non-GFP form of BBa_K2623023. It's also another form of BBa_K2623022 in which SpyTag was inserted to OmpA protein's C-termini, designed to target our archaeal ribosomal protein protein L7Ae and then siRNA into outer-membrane vesicles (OMVs). Porin outer-membrane protein A, OmpA, is a kind of highly expressed transmembrane porin protein of E. coli and is therefore abundant in OMVs. We inserted SpyTag to its C-termini and SpyCather to L7Ae (<partinfo>BBa_K2623026</partinfo>) in order to anchore L7Ae to outer membrane through the bio-conjugation of the SpyTag/SpyCatcher system.
+This part is the non-GFP form of <partinfo>BBa_K2623023</partinfo>. It's also another form of <partinfo>BBa_K2623022</partinfo> in which SpyTag is inserted to the C-termini of OmpA and is designed to target our archaeal ribosomal protein protein L7Ae and then siRNA into outer-membrane vesicles (OMVs). Porin outer-membrane protein A, OmpA, is a kind of highly expressed transmembrane porin protein of E. coli and is therefore abundant in OMVs. We insert SpyTag to its C-termini and SpyCather to L7Ae (<partinfo>BBa_K2623026</partinfo>) in order to anchore L7Ae to outer membrane through the bio-conjugation of the SpyTag/SpyCatcher system.
 <!-- Add more about the biology of this part here