Difference between revisions of "Part:BBa K2632002"
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− | Gene <i>sifA</i> locates in <i>Salmonella</i> pathogenicity island-2 (SPI-2) and has the function of maintaining the integrity of <i>Salmonella</i>-Containing Vacuole (SCV). Some studies was reported that expression of SifA is required for intracellular enviroment. In other words, promoter of <i>sifA</i> expresses SifA only when salmonella enter the host cells <sup>1</sup>. A fragment including sifA promoter region and RBS region were amplified from Salmonella SL1344 genome. | + | Gene <i>sifA</i> locates in <i>Salmonella</i> pathogenicity island-2 (SPI-2) and has the function of maintaining the integrity of <i>Salmonella</i>-Containing Vacuole (SCV). Some studies was reported that expression of SifA is required for intracellular enviroment. In other words, promoter of <i>sifA</i> expresses SifA only when <i>salmonella</i> enter the host cells <sup>1</sup>. A fragment including sifA promoter region and RBS region were amplified from <i>Salmonella</i> SL1344 genome. |
SipD is required for bacterial internalization. ΔsipD mutant can not enter host cells. Thus, we use this strain as a control. Microscopy suggests that only intracellular bacteria express eGFP which under the control of P<sub style:"font-family: italic">sifA</sub> (Figure 1). | SipD is required for bacterial internalization. ΔsipD mutant can not enter host cells. Thus, we use this strain as a control. Microscopy suggests that only intracellular bacteria express eGFP which under the control of P<sub style:"font-family: italic">sifA</sub> (Figure 1). | ||
<img style="width: 800px; margin-left: 50px" src="https://static.igem.org/mediawiki/2018/9/90/T--HZAU-China--PsifA.jpg"> | <img style="width: 800px; margin-left: 50px" src="https://static.igem.org/mediawiki/2018/9/90/T--HZAU-China--PsifA.jpg"> |
Revision as of 12:24, 13 October 2018
Promoter sifA
1. Garmendia, J., Beuzon, C. R., Ruiz-Albert, J. & Holden, D. W. The roles of SsrA-SsrB and OmpR-EnvZ in the regulation of genes encoding the Salmonella typhimurium SPI-2 type III secretion system. Microbiology 149, 2385-2396, doi:10.1099/mic.0.26397-0 (2003).
Figure 1. Hela GSDMD KO cells respectively infected with the ΔsipD or ΔsifA mutant. These mutants contain a low copy number plasmid to express eGFP which was regulated by PsifA.
Notice
This part should be in a low copy plasmid such as PSB4K5, or it will express before Salmonella's entry to cell.
Use in Salmonella only.
Reference
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]