Difference between revisions of "Part:BBa K2623021"
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<partinfo>BBa_K2623021 short</partinfo>
 
<partinfo>BBa_K2623021 short</partinfo>
 
===SUMMARY===
 
===SUMMARY===
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This part is a relatively basic part designed to target the archaeal ribosomal protein protein L7Ae and then siRNA into outer-membrane vesicles (OMVs). Porin outer-membrane protein A, OmpA, is a kind of highly expressed transmembrane porin protein of E. coli and is therefore abundant in OMVs. We inserted SpyTag to its N-termini to bioconjugate with the SpyCather in the following parts. to L7Ae (BBa_K2623006) in order to anchore L7Ae to outer membrane through the bioconjugation of the SpyTag/SpyCatcher system. Through the fluorescence of GFP, we can evaluate the efficiency of their transport to the outer membrane and OMVs.
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This part is a relatively basic part designed to target the archaeal ribosomal protein protein L7Ae and then siRNA into outer-membrane vesicles (OMVs). Porin outer-membrane protein A, OmpA, is a kind of highly expressed transmembrane porin protein of E. coli and is therefore abundant in OMVs. We inserted SpyTag to its N-termini to bioconjugate with the SpyCather in the following parts.  
 +
In order to test the efficiency of anchoring the OmpA-SpyTag (OmpA-ST) in our circuit, we use GFP, <partinfo>BBa_I13401</partinfo> as the reporter, because the fluorescence intensity is easier to be measured.
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 07:38, 13 October 2018


Bacterial outer membrane protein A (OmpA) fused with SpyTag at its N-termini and GFP at its C-termin

SUMMARY

This part is a relatively basic part designed to target the archaeal ribosomal protein protein L7Ae and then siRNA into outer-membrane vesicles (OMVs). Porin outer-membrane protein A, OmpA, is a kind of highly expressed transmembrane porin protein of E. coli and is therefore abundant in OMVs. We inserted SpyTag to its N-termini to bioconjugate with the SpyCather in the following parts. In order to test the efficiency of anchoring the OmpA-SpyTag (OmpA-ST) in our circuit, we use GFP, BBa_I13401 as the reporter, because the fluorescence intensity is easier to be measured.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 65
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1302