Difference between revisions of "Part:BBa K2680116"

Revision as of 21:51, 13 October 2018

3G pLac/ara-1

pLac/ara-1 dual input promoter in a 3G-compatible form. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

Flanked by prefix sticky end A and suffix sticky end B. Repressed by LacI and activated by arabinose/AraC. Intended for use in oscillator creation. Sequence originally from Lutz and Bujard 1997; later used for the creation of oscillator from Stricker et al 2008. Note that the upstream LacO site is required for functional repression via LacI (tetrameter) [See Stricker et al 2008 supplement].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 476
Illegal XhoI site found at 443
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 41
Illegal BsaI.rc site found at 556

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 <partinfo>BBa_K2680116 short</partinfo>
-G-compatible pLac/ara-1 dual input promoter. Flanked by prefix sticky end A and suffix sticky end B. Repressed by LacI and activated by arabinose/AraC. Intended for use in oscillator creation. Sequence originally from Lutz and Bujard 1997; later used for the creation of oscillator from Stricker et al 2008. Note that the upstream LacO site is required for functional repression via LacI (tetrameter) [See Stricker et al 2008 supplement].
+pLac/ara-1 dual input promoter in a 3G-compatible form. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].
+Flanked by prefix sticky end A and suffix sticky end B. Repressed by LacI and activated by arabinose/AraC. Intended for use in oscillator creation. Sequence originally from Lutz and Bujard 1997; later used for the creation of oscillator from Stricker et al 2008. Note that the upstream LacO site is required for functional repression via LacI (tetrameter) [See Stricker et al 2008 supplement].
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