Difference between revisions of "Part:BBa K2686006"

Revision as of 14:56, 16 October 2018

Encapsulin protein with HexaHistidine insert, OT1 peptide at C terminus and a sfGFP-tag protein.

This Composite part consists of an encapsulin protein with a HexaHistidine linker and an OT1 peptide fused to the C terminus, followed by a T7 RBS and a sfGFP protein with a 15 amino acid long peptide tag. This peptide tag allows the sfGFP to bind to the encapsulin's interior surface, thus allowing the loading of sfGFP in the Encapsulin multimer. This would allow having a fluorescent Encapsulin protein.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 902
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 77
Illegal BglII site found at 492
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 902
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 902
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 426
Illegal SapI.rc site found at 457
Illegal SapI.rc site found at 955

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2686006 short</partinfo>
-This Composite part consists of an encapsulin protein with a HexaHistidine linker and an OVA peptide fused to the C terminus, followed by a T7 RBS and a sfGFP protein with a 15 amino acid long peptide tag. This peptide tag allows the sfGFP to bind to the encapsulin's interior surface, thus allowing the loading of sfGFP in the Encapsulin multimer. This would allow having a fluorescent Encapsulin protein.
+This Composite part consists of an encapsulin protein with a HexaHistidine linker and an OT1 peptide fused to the C terminus, followed by a T7 RBS and a sfGFP protein with a 15 amino acid long peptide tag. This peptide tag allows the sfGFP to bind to the encapsulin's interior surface, thus allowing the loading of sfGFP in the Encapsulin multimer. This would allow having a fluorescent Encapsulin protein.
 <!-- Add more about the biology of this part here