Difference between revisions of "Part:BBa K2656100"
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<li></html>[https://parts.igem.org/Part:BBa_K2656004 BBa_K2656004]: the [https://parts.igem.org/Part:BBa_J23106 J23106] promoter in its Golden Braid compatible version <html></li> | <li></html>[https://parts.igem.org/Part:BBa_K2656004 BBa_K2656004]: the [https://parts.igem.org/Part:BBa_J23106 J23106] promoter in its Golden Braid compatible version <html></li> | ||
<li></html>[https://parts.igem.org/Part:BBa_K2656008 BBa_K2656008]: the [https://parts.igem.org/Part:BBa_B0030 B0030] ribosome biding site in its Golden Braid compatible version | <li></html>[https://parts.igem.org/Part:BBa_K2656008 BBa_K2656008]: the [https://parts.igem.org/Part:BBa_B0030 B0030] ribosome biding site in its Golden Braid compatible version | ||
| − | <li></html> [https://parts.igem.org/Part:BBa_K2656013 BBa_K2656013]: [https://parts.igem.org/Part:BBa_I746916 BBa_I746916] sfGFP sequence in its Golden Braid standardized version <html></li> | + | <li></html> [https://parts.igem.org/Part:BBa_K2656013 BBa_K2656013]: the [https://parts.igem.org/Part:BBa_I746916 BBa_I746916] sfGFP sequence in its Golden Braid standardized version <html></li> |
<li></html> Terminator [https://parts.igem.org/Part:BBa_K2656026 BBa_K2656026]: the [https://parts.igem.org/Part:BBa_B0015 B0015] transcriptional terminator in its Golden Braid compatible version<html></li> | <li></html> Terminator [https://parts.igem.org/Part:BBa_K2656026 BBa_K2656026]: the [https://parts.igem.org/Part:BBa_B0015 B0015] transcriptional terminator in its Golden Braid compatible version<html></li> | ||
</ul> | </ul> | ||
Revision as of 17:47, 12 October 2018
sfGFP transcriptional unit 1
Transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease.
This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:
- BBa_K2656004: the J23106 promoter in its Golden Braid compatible version
- BBa_K2656008: the B0030 ribosome biding site in its Golden Braid compatible version
- </html> BBa_K2656013: the BBa_I746916 sfGFP sequence in its Golden Braid standardized version
- Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 11
Illegal NheI site found at 34 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 77