Difference between revisions of "Part:BBa K2656100"
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<li></html>[https://parts.igem.org/Part:BBa_K2656008 BBa_K2656008]: the [https://parts.igem.org/Part:BBa_B0030 B0030] ribosome biding site in its Golden Braid compatible version  
 
<li></html>[https://parts.igem.org/Part:BBa_K2656008 BBa_K2656008]: the [https://parts.igem.org/Part:BBa_B0030 B0030] ribosome biding site in its Golden Braid compatible version  
 
<li></html>[https://parts.igem.org/Part:BBa_K2656013 BBa_K2656013]: [https://parts.igem.org/Part:BBa_I746916 BBa_I746916]  sfGFP sequence in its Golden Braid standardized version<html></li>
 
<li></html>[https://parts.igem.org/Part:BBa_K2656013 BBa_K2656013]: [https://parts.igem.org/Part:BBa_I746916 BBa_I746916]  sfGFP sequence in its Golden Braid standardized version<html></li>
−
<li></html>[https://parts.igem.org/Part:]: the [https://parts.igem.org/Part:BBa_B0015 B0015] transcriptional terminator in its Golden Braid compatible version<html></li>
+
<li></html>Terminator  [https://parts.igem.org/Part:BBa_K2656026 BBa_K2656026]: the [https://parts.igem.org/Part:BBa_B0015 B0015] transcriptional terminator in its Golden Braid compatible version<html></li>
 
</ul>
 
</ul>
 
</html>
 
</html>

Revision as of 17:24, 12 October 2018


sfGFP transcriptional unit 1

Transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease.

This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 77