Difference between revisions of "Part:BBa K2686005"
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<partinfo>BBa_K2686005 short</partinfo>
 
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This Encapsulin protein was first modified by insertion of a hexahistidine insert to increase heat stability, and endow better hydrodynamic properties. After the encapsulin site on the plasmid (After the C-terminus), is a site for sfGFP under native promoter included within BSAI cut sites. These BSAI cut sites would allow for the rapid, scarless introduction of antigen peptides which will be expressed on the surface of the Encapsulin subunit (After the C-terminus). The insert in between the two BsaI cut sites, consisting of sfGFP with a native promoter and terminator, allows for checking the success of the insertion of the antigen (green colonies do not contain the desired peptide insert).
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Composite part designed to serve as a rapid platform for fusing antigen coding sequences to the C-terminus of the encapsulin protein.
  
 
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===Usage and Biology===
 
===Usage and Biology===
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This Encapsulin protein was first modified by insertion of a hexahistidine insert to increase heat stability, and endow better hydrodynamic properties. After the encapsulin site on the plasmid (After the C-terminus), is a site for sfGFP under native promoter included within BsaI cut sites. These BsaI cut sites would allow for the rapid, scarless introduction of antigen peptides which will be expressed on the surface of the Encapsulin subunit (After the C-terminus). The insert in between the two BsaI cut sites, consisting of sfGFP with a native promoter and terminator, allows for checking the success of the insertion of the antigen (green colonies do not contain the desired peptide insert).
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2686005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2686005 SequenceAndFeatures</partinfo>
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Revision as of 13:15, 14 October 2018


Encapsulin protein with HexaHistidine insert & sfGFP under native promoter included between BsaI cut

Composite part designed to serve as a rapid platform for fusing antigen coding sequences to the C-terminus of the encapsulin protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 77
    Illegal BglII site found at 492
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 426
    Illegal SapI.rc site found at 457
    Illegal SapI.rc site found at 1031