Difference between revisions of "Part:BBa K2559011"
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<partinfo>BBa_K2559011 short</partinfo>
 
<partinfo>BBa_K2559011 short</partinfo>
  
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It is a weak promoter found in E.coli, and we uesd it to express our cording gene regulatedly， the expression of target genes is low and avoid the risk of losing transformed algae strains for toxic effect caused by target genes.
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The BBa_K2559011 , caiF promoter is a endogenous promoter in Escherichia coli K 12
 
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===Usage and Biology===
 
===Usage and Biology===
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We have obtained this promoter from a database, PromEC ( http://margalit.huji.ac.il/promec/index.html) .PromEC is an updated compilation of E. coli mRNA promoter sequences. It includes documentation on the location of experimentally identified mRNA transcriptional start sites on the E. coli chromosome, as well as the actual sequences in the promoter region. The database is currently updated as of July 2000 and includes 471 entries.
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We have built up a model to measure the expression strength  of these promoters and tested the prplJ, pdapA, and pcaiF expression strength by comparing the fluorescent intensity.You can use PromEC  and our model to obtain more useful promoter information.
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The eGFP expression in E.coli(DH5 alpha) and was driven by prplj/pdapa/pcaif promoter.
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[[File:Scau-china-2018-11.png|800px|thumb|center|Figure1 The result of the fluorescent intensity measurement ]]
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[[File:Scau-china-2018-12.png|800px|thumb|center|Figure2 Fluorescent intensity of eGFP driven by by rplj/dapa/caif promoter.]]
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The part is facilitate the in-depth research for other teams!
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2559011 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2559004 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2559011 parameters</partinfo>
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<partinfo>BBa_K2559004 parameters</partinfo>
 
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Revision as of 07:05, 15 October 2018


PcaiF promoter

The BBa_K2559011 , caiF promoter is a endogenous promoter in Escherichia coli K 12 Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]