Difference between revisions of "Part:BBa K2789001:Design"
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| − | We have condon-optimeized Ccas BBa_K2789009 for E.coli from BBa_K608102 and deleted the EcoRI and SpeI site in the gene sequence. We paste the website of BBa_K608102 (https://parts.igem.org/Part:BBa_K608102) | + | We have condon-optimeized Ccas BBa_K2789009 for E.coli from BBa_K608102 and deleted the EcoRI and SpeI site in the gene sequence. We paste the website of BBa_K608102 (https://parts.igem.org/Part:BBa_K608102:Design) |
And this part BBa_K2789001 is consists of lac operator , Ccas and Ccar (The downstream signal transducers to control gene expression) | And this part BBa_K2789001 is consists of lac operator , Ccas and Ccar (The downstream signal transducers to control gene expression) | ||
Revision as of 05:49, 12 October 2018
lac operator+rbs+Ccas+terminator+Ccar+lac operator+rbs+terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1393
Illegal BamHI site found at 2973 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 798
Illegal AgeI site found at 1803
Illegal AgeI site found at 1998 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We have optimized the code in the Ccas and Ccar. And we deleted the EcoRI restriction site in the Ccas for future DNA ligation and digestion.
Source
All the parts come from igem registry resources.
Ccas: BBa_K608102
Ccar: BBa_K592002
Lac operator: BBa_C0010, BBa_C0012
double terminator: BBa_B0015
rbs: BBa_B0034
References
We have condon-optimeized Ccas BBa_K2789009 for E.coli from BBa_K608102 and deleted the EcoRI and SpeI site in the gene sequence. We paste the website of BBa_K608102 (https://parts.igem.org/Part:BBa_K608102:Design)
And this part BBa_K2789001 is consists of lac operator , Ccas and Ccar (The downstream signal transducers to control gene expression)