Difference between revisions of "Part:BBa K2694000:Design"
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===Design Notes=== | ===Design Notes=== | ||
This circuit was designed to produce the biobrick <bbpart>BBa_K2694001</bbpart>. The IPTG inducible LacI promoter and a strong ribosome binding site. A double terminator was used at the end of the circuit to prevent continued read-through. | This circuit was designed to produce the biobrick <bbpart>BBa_K2694001</bbpart>. The IPTG inducible LacI promoter and a strong ribosome binding site. A double terminator was used at the end of the circuit to prevent continued read-through. | ||
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===Source=== | ===Source=== | ||
| − | Wilhelm et al. | + | Wilhelm et al. (1999). Synthesized through BioBasic. |
===References=== | ===References=== | ||
| + | Wilhelm S., Thomassen J., & Jaeger K.E. (1999). A Novel Lipolytic Enzyme Located in the Outer Membrane of Pseudomonas aeruginosa. Journal of Bacteriology. 6977-86. | ||
Latest revision as of 00:48, 12 October 2018
EstA generator under control of pLac promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 296
Illegal BglII site found at 443 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 226
Illegal NgoMIV site found at 682
Illegal NgoMIV site found at 820
Illegal NgoMIV site found at 1360
Illegal NgoMIV site found at 1729 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This circuit was designed to produce the biobrick BBa_K2694001. The IPTG inducible LacI promoter and a strong ribosome binding site. A double terminator was used at the end of the circuit to prevent continued read-through.
Source
Wilhelm et al. (1999). Synthesized through BioBasic.
References
Wilhelm S., Thomassen J., & Jaeger K.E. (1999). A Novel Lipolytic Enzyme Located in the Outer Membrane of Pseudomonas aeruginosa. Journal of Bacteriology. 6977-86.