Difference between revisions of "Part:BBa K2694000"

Latest revision as of 00:19, 12 October 2018

EstA generator under control of pLac promoter

The esterase in this circuit is an enzyme from Pseudomonas aeruginosa. The function of the esterase is to cleave ester bonds, such as those found in triglycerides. The production of the protein is under the control of a pLac promoter (BBa_R0011), with a strong ribosome binding site (BBa_B0034). At the end of the circuit there is a double terminator (BBa_B0010 and BBa_B0012). The circuit was designed by the 2018 NDC-HighRiverAB team to produce the esterase protein for testing purposes. Our team introduced this circuit to DH5α and tested its activity towards two different substrates (4-nitrophenyl palmitate and 4-nitrophenyl octanoate).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 296
Illegal BglII site found at 443
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 226
Illegal NgoMIV site found at 682
Illegal NgoMIV site found at 820
Illegal NgoMIV site found at 1360
Illegal NgoMIV site found at 1729
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2694000 short</partinfo>
-The esterase in this circuit is an enzyme from Pseudomonas aeruginosa. The function of the esterase is to cleave ester bonds, such as those found in triglycerides. The production of the protein is under the control of a pLac promoter (R0011), with a strong ribosome binding site (B0034). At the end of the circuit there is a double terminator (B0010 and B0012). The circuit was designed by the 2018 NDC-HighRiverAB team to produce the esterase protein for testing purposes. Our team introduced this circuit to DH5&alpha; and tested its activity towards two different substrates (4-nitrophenyl palmitate and 4-nitrophenyl octanoate).
+The esterase in this circuit is an enzyme from Pseudomonas aeruginosa. The function of the esterase is to cleave ester bonds, such as those found in triglycerides. The production of the protein is under the control of a pLac promoter (<bbpart>BBa_R0011</bbpart>), with a strong ribosome binding site (<bbpart>BBa_B0034</bbpart>). At the end of the circuit there is a double terminator (<bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>). The circuit was designed by the 2018 NDC-HighRiverAB team to produce the esterase protein for testing purposes. Our team introduced this circuit to DH5&alpha; and tested its activity towards two different substrates (4-nitrophenyl palmitate and 4-nitrophenyl octanoate).
 <!-- Add more about the biology of this part here