Difference between revisions of "Part:BBa K2787029"

Revision as of 09:29, 11 October 2018

RNA attenuator pT181 with GFP under pT181 target sequence by Kyoto 2013 iGEM Team

Measurement of repression efficiency of pT181 sense target submitted by Kyoto 2013 iGEM team(BBa_K1126003) under the expression kit(BBa_K2787027) we designed to test the functionality of our improved version of pT181 sense target(BBa_K2787030). This part is identical to BBa_K2787027 except for the pT181 sense target is changed to BBa_K1126003 and a RBS(BBa_K1893033).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 268
Illegal NheI site found at 291
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1247

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 <partinfo>BBa_K2787029 short</partinfo>
-pT181 is a RNA switch that can repress the expression of target gene by binding its antisense RNA to its sense target DNA placed upstream of the coding sequenced of target gene. Here the part carries a pT181 Antisense under constitutive promoter J23100 and a GFP reporter whose coding sequence is placed seamlessly after pT181 sense target, also under J23100. This part is used for testing the repressive efficiency of pT181 efficiency by contrast to the results from part BBa_K2787028.
+Measurement of repression efficiency of pT181 sense target submitted by Kyoto 2013 iGEM team(BBa_K1126003) under the expression kit(BBa_K2787027) we designed to test the functionality of our improved version of pT181 sense target(BBa_K2787030). This part is identical to BBa_K2787027 except for the pT181 sense target is changed to BBa_K1126003 and a RBS(BBa_K1893033).
 <!-- Add more about the biology of this part here