Difference between revisions of "Part:BBa K2788001"
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<center><html><img src='https://static.igem.org/mediawiki/2018/e/e9/T--SZU-China--Result_6.png' style="width:60%;margin:0 auto"> | <center><html><img src='https://static.igem.org/mediawiki/2018/e/e9/T--SZU-China--Result_6.png' style="width:60%;margin:0 auto"> | ||
| − | <center>Fig.1 Construction of expression vector Bbchit-pBC. PgpdA and TtrpC come from parts of 2016_NYMU-Taipei: BBa_K2040101 and BBa_K2040102, and Bbchit comes from the Beauveria bassiana ARSEF 2860. The PgpdA-Bbchit-TtrpC part is connected to the pBC plasmid through the BioBrick site.</center></html></center> | + | <center>Fig.1 Construction of expression vector Bbchit-pBC. PgpdA and TtrpC come from parts of 2016_NYMU-Taipei: BBa_K2040101 and BBa_K2040102, and Bbchit comes from the Beauveria bassiana ARSEF 2860. The PgpdA-Bbchit-TtrpC part is connected to the pBC plasmid through the BioBrick site.</center></html></center> |
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Revision as of 06:38, 11 October 2018
Bbchit_Beauveria bassiana ARSEF 2860
This part is the coding sequence (CDS) of Chitinase from The Beauveria bassiana ARSEF 2860(GenBank Acc.No.txid655819). It can encode chitinase and effectively decomposes chitin. We transferred this gene to enhance the penetration of Metarhizium anisopliae.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 581
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 568
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 250
Illegal NgoMIV site found at 779 - 1000COMPATIBLE WITH RFC[1000]
iGEM2018 SZU-China
In order to make Metarhizium anisopliae penetrate the corpus callosum more efficiently, we constructed an expression vector containing part Bbchit(Fig.1)
