Difference between revisions of "Part:BBa K2683030"

Revision as of 02:31, 12 October 2018

Peptidyl Prolyl Isomerase (PPiase) in pSB1C3

Peptidyl-prolyl isomerase (PPiase) is a protein that aids the isomerization of prolines in order to catalyze the addition proline amino acids onto the peptide chains from the aminoacyl-tRNA it is attached to [1]. This part has been codon optimized for use in E.coli , Contains a N-terminal his tag, and has a T7 promoter, medium strength RBS, and double terminator. This part was originally designed by the 2017 LEthbridge iGEm team (http://2017.igem.org/Team:Lethbridge).

This part was improved by placing it into the pSB1C3 plasmid.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2683030 short</partinfo>
-PPiase in pSb1C3
+Peptidyl-prolyl isomerase (PPiase) is a protein that aids the isomerization of prolines in order to catalyze the addition proline amino acids onto the peptide chains from the aminoacyl-tRNA it is attached to [1].  This part has been codon optimized for use in <i> E.coli </i>, Contains a N-terminal his tag, and has a T7 promoter, medium strength RBS, and double terminator. This part was originally designed by the 2017 LEthbridge iGEm team (http://2017.igem.org/Team:Lethbridge).
+This part was improved by placing it into the pSB1C3 plasmid.
 <!-- Add more about the biology of this part here