Difference between revisions of "Part:BBa K2665014"

Revision as of 03:29, 11 October 2018

TDH3 promoter->SseNHX1->6xHis->CYC terminator

SseNHX1

Usage and Biology

SseNHX1 is the vacuolar Na(+)/H(+) antiporter. This antiporter enables to survive high osmotic pressure by sequestering excessive cytoplasmic Na(+) into vacuole.The gene, SseNHX1 is created by DNA family shuffling; Two homologous Na(+)/H(+) antiporters from halophytes Salicornia europaea (SeNHX1) and Suaeda salsa (SsNHX1) were shuffled. By reference, SseNHX1 enables higher salt tolerance and sequester Na(+) than the two protein, SeNHX1 and SsNHX1.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 2379
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <!-- Add more about the biology of this part here-->
 ===Usage and Biology===
-SseNHX1 is the vacuolar Na(+)/H(+) antiporter. This antiporter enables to survive high osmotic pressure by sequestering excessive cytoplasmic Na(+) into vacuole.
+SseNHX1 is the vacuolar Na(+)/H(+) antiporter. This antiporter enables to survive high osmotic pressure by sequestering excessive cytoplasmic Na(+) into vacuole.The gene, SseNHX1 is created by DNA family shuffling;  Two homologous Na(+)/H(+) antiporters from halophytes Salicornia europaea (SeNHX1) and Suaeda salsa (SsNHX1) were shuffled. By reference, SseNHX1 enables higher salt tolerance and sequester Na(+) than the two protein, SeNHX1 and SsNHX1.
- The gene, SseNHX1 is created by DNA family shuffling;  Two homologous Na(+)/H(+) antiporters from halophytes Salicornia europaea (SeNHX1) and Suaeda salsa (SsNHX1) were shuffled.
-By reference, SseNHX1 enables higher salt tolerance and sequester Na(+) than the two protein, SeNHX1 and SsNHX1.