Difference between revisions of "Part:BBa K2617017"
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<partinfo>BBa_K2617017 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2617017 SequenceAndFeatures</partinfo>
  
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==Characterization==
 
We have improved the previous part [https://parts.igem.org/Part:BBa_J23100 BBa_J23100] by adding RBS and pelB-5D to make it can be used for extracelluar expression. The combination of promoter, RBS and signal peptide makes it convenient to use as a biobrick and gives it the function of extracellular expression.
 
We have improved the previous part [https://parts.igem.org/Part:BBa_J23100 BBa_J23100] by adding RBS and pelB-5D to make it can be used for extracelluar expression. The combination of promoter, RBS and signal peptide makes it convenient to use as a biobrick and gives it the function of extracellular expression.
  

Revision as of 03:05, 11 October 2018


J23100-RBS-pelB-5D

A combination system of promoter, ribosome binding site and signal peptide for enhancing protein expression(pelB)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 115
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

We have improved the previous part BBa_J23100 by adding RBS and pelB-5D to make it can be used for extracelluar expression. The combination of promoter, RBS and signal peptide makes it convenient to use as a biobrick and gives it the function of extracellular expression.