Difference between revisions of "Part:BBa K2692001:Design"
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
The addition of a his tag for purification purposes
+
The coding sequence has been optimized for use in <i> E.coli </i>
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+
−
 
+
  
 
===Source===
 
===Source===

Revision as of 02:33, 11 October 2018


Major T4 Capsid Protein gp23


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9
    Illegal PstI site found at 342
    Illegal PstI site found at 1524
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal PstI site found at 342
    Illegal PstI site found at 1524
    Illegal NotI site found at 15
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
    Illegal BamHI site found at 1069
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9
    Illegal PstI site found at 342
    Illegal PstI site found at 1524
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9
    Illegal XbaI site found at 24
    Illegal PstI site found at 342
    Illegal PstI site found at 1524
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1118


Design Notes

The coding sequence has been optimized for use in E.coli

Source

The part was synthesized as a g-block (linear Piece of DNA) through IDT.

References

[1]Olson N., Gingery, M., Eiserling, F., and Baker, T. (2001) The structure of isometric capsids of bacteriphage T4. Virology. 279,385-391