Difference between revisions of "Part:BBa K2692001:Design"
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===References=== | ===References=== | ||
− | [1] | + | [1]Olson N., Gingery, M., Eiserling, F., and Baker, T. (2001) The structure of isometric capsids of bacteriphage T4. Virology. 279,385-391 |
Revision as of 02:31, 11 October 2018
Major T4 Capsid Protein gp23
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 9
Illegal PstI site found at 342
Illegal PstI site found at 1524 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 9
Illegal PstI site found at 342
Illegal PstI site found at 1524
Illegal NotI site found at 15 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 9
Illegal BamHI site found at 1069 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 9
Illegal PstI site found at 342
Illegal PstI site found at 1524 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 9
Illegal XbaI site found at 24
Illegal PstI site found at 342
Illegal PstI site found at 1524 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1118
Design Notes
The addition of a his tag for purification purposes
Source
The part was synthesized as a g-block (linear Piece of DNA) through IDT.
References
[1]Olson N., Gingery, M., Eiserling, F., and Baker, T. (2001) The structure of isometric capsids of bacteriphage T4. Virology. 279,385-391