Difference between revisions of "Part:BBa K2627006:Design"
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+ | Chang Y, Su T, Qi Q*, Liang Q*. Easy regulation of metabolic flux in Escherichia coli using an endogenous type I-E CRISPR-Cas system. Microb Cell Fact. 2016 Nov 15;15(1):195.. |
Revision as of 02:52, 11 October 2018
paraBAD-crRNA targeting gltA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 253
Illegal XhoI site found at 365 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 88
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 70
Design Notes
...
Source
Easy regulation of metabolic flux in Escherichia coli using an endogenous type I-E CRISPR-Cas system
References
Chang Y, Su T, Qi Q*, Liang Q*. Easy regulation of metabolic flux in Escherichia coli using an endogenous type I-E CRISPR-Cas system. Microb Cell Fact. 2016 Nov 15;15(1):195..