Difference between revisions of "Part:BBa K2633006:Experience"
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===Applications of BBa_K2633006=== | ===Applications of BBa_K2633006=== | ||
This part was transformed using a restriction site illegal version of BBa_K2633000[https://parts.igem.org/Part:BBa_K2633000]. It was then used to inoculate ''Brachypodium distachyon'' seeds. Transformed bacteria expressing GFP were observed on and inside roots from 1 to 4 weeks post inoculation. | This part was transformed using a restriction site illegal version of BBa_K2633000[https://parts.igem.org/Part:BBa_K2633000]. It was then used to inoculate ''Brachypodium distachyon'' seeds. Transformed bacteria expressing GFP were observed on and inside roots from 1 to 4 weeks post inoculation. | ||
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Figure 1. A) FCP2-01 transformed with GFP (left) vs wild-type (right) under blue light with orange filter. B) Transformed colonies on an agar plate. | Figure 1. A) FCP2-01 transformed with GFP (left) vs wild-type (right) under blue light with orange filter. B) Transformed colonies on an agar plate. | ||
Revision as of 00:56, 11 October 2018
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Applications of BBa_K2633006
This part was transformed using a restriction site illegal version of BBa_K2633000[1]. It was then used to inoculate Brachypodium distachyon seeds. Transformed bacteria expressing GFP were observed on and inside roots from 1 to 4 weeks post inoculation.
Figure 1. A) FCP2-01 transformed with GFP (left) vs wild-type (right) under blue light with orange filter. B) Transformed colonies on an agar plate.
Figure 2. 3 weeks post inoculation on plant roots. A) FCP2-01 forming large colonies abount 2 mm long in B. distachyon roots B) Detail of panel A. C) FCP2-01 growing between plant cells on the root surface. D) Detail of colonies on root epidermis.
Figure 3. 4 weeks post inoculation on plant roots. A) FCP2-01 on B. distachyon roots. B) FCP2-0 colonizing root hairs. C) FCP2-01 growing on epidermis of the root. D) Enlarged image of panel C.
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