Difference between revisions of "Part:BBa K2719013:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Blue | + | This was mainly created with the idea of making a control for collagen to see how a combination of reporter proteins would behave. For this we used to promoters, one for each protein (in the case of RFP it is a tetracycline repressible promoter); RFP and Blue chromoprotein coding sequences and double terminator for each one of the reporter proteins. In addition, this part was modify so it could be compatible with pSB1C3 plasmid, RFC [10] and <i>E. coli</i> BL21 (DE3). |
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===Source=== | ===Source=== | ||
Revision as of 13:20, 17 October 2018
Blue Chromoprotein and RFP combined reporter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 938
Illegal NheI site found at 961 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 635
Illegal AgeI site found at 747 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This was mainly created with the idea of making a control for collagen to see how a combination of reporter proteins would behave. For this we used to promoters, one for each protein (in the case of RFP it is a tetracycline repressible promoter); RFP and Blue chromoprotein coding sequences and double terminator for each one of the reporter proteins. In addition, this part was modify so it could be compatible with pSB1C3 plasmid, RFC [10] and E. coli BL21 (DE3).
Source
Blue Chromoprotein and RFP combined reporter