Difference between revisions of "Part:BBa K2694000:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
-
+
This circuit was designed to produce the biobrick <bbpart>BBa_K2694001</bbpart>. The IPTG inducible LacI promoter and a strong ribosome binding site. A double terminator was used at the end of the circuit to prevent continued read-through.
  
  
  
−
===Source===
 
  
−
-
+
===Source===
 +
Wilhelm et al., J Bacteriol, 1999, 6977-86 and BBa_K2694000. Synthesized through BioBasic.
  
 
===References===
 
===References===

Revision as of 00:40, 12 October 2018


EstA generator under control of pLac promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 296
    Illegal BglII site found at 443
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 226
    Illegal NgoMIV site found at 682
    Illegal NgoMIV site found at 820
    Illegal NgoMIV site found at 1360
    Illegal NgoMIV site found at 1729
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This circuit was designed to produce the biobrick BBa_K2694001. The IPTG inducible LacI promoter and a strong ribosome binding site. A double terminator was used at the end of the circuit to prevent continued read-through.



Source

Wilhelm et al., J Bacteriol, 1999, 6977-86 and BBa_K2694000. Synthesized through BioBasic.

References