Difference between revisions of "Part:BBa K2668010:Design"
 
Line 8: Line 8:
 
===Design Notes===
 
===Design Notes===
 
<html>
 
<html>
−
The part BBa_K2668010 comes from the <a href="http://2018.igem.org/Team:Toulouse-INSA-UPS/Design">iGEM Team Toulouse-INSA-UPS Cerberus Project</a>. It was design for binding cellulose to different types of compounds at the same time thanks to its two very unlike linkers. The streptavidin linker can bind biotin and biotinylated compounds while the UnAA-containing linker will click molecules that bear an alkyne function.
+
The part BBa_K2668010 comes from the <a href="http://2018.igem.org/Team:Toulouse-INSA-UPS/Design">iGEM Team Toulouse-INSA-UPS Cerberus Project</a>. It was originally designed to bind a variety of compounds to cellulose thanks to its two linkers. The streptavidin linker can bind biotin and biotinylated compounds while the UnAA-containing linker will click molecules that bear an alkyne function.
 
</html>
 
</html>
  
Line 14: Line 14:
 
===Source===
 
===Source===
  
−
The Part was obtained by IDT gBlock synthesis. The CBM3a domain is a corrected version of the Part:BBa_K1321014, RFC10 compatible . The monomeric streptavidin is the Part:BBa_K1896000.
+
The Part was obtained by IDT gBlock synthesis. The CBM3a domain is a corrected version of the Part:BBa_K1321014, RFC10 compatible. The monomeric streptavidin is the Part:BBa_K1896000.
  
  
  
 
===References===
 
===References===

Latest revision as of 14:00, 13 October 2018


Cerberus : A Molecular Binding Plateform (mSA2-CBM3a-AzF)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 358
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part BBa_K2668010 comes from the iGEM Team Toulouse-INSA-UPS Cerberus Project. It was originally designed to bind a variety of compounds to cellulose thanks to its two linkers. The streptavidin linker can bind biotin and biotinylated compounds while the UnAA-containing linker will click molecules that bear an alkyne function.


Source

The Part was obtained by IDT gBlock synthesis. The CBM3a domain is a corrected version of the Part:BBa_K1321014, RFC10 compatible. The monomeric streptavidin is the Part:BBa_K1896000.


References