Difference between revisions of "Part:BBa K2549028"
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This part is one of the response elements of our amplifier, also executing the combiner function. 8*ZF43.8 binding sites is assembled using two 4*ZF43.8 binding sites ([[Part:BBa_K2446006]]) with a biobrick scar between them. Minimal CMV ([[Part:BBa_K2549049]]) is a promotor providing very low basal expression and high maximal expression after induction. This part can switch on the expression of gene downstream after induced by our zinc finger-based transcription activator.
 
This part is one of the response elements of our amplifier, also executing the combiner function. 8*ZF43.8 binding sites is assembled using two 4*ZF43.8 binding sites ([[Part:BBa_K2446006]]) with a biobrick scar between them. Minimal CMV ([[Part:BBa_K2549049]]) is a promotor providing very low basal expression and high maximal expression after induction. This part can switch on the expression of gene downstream after induced by our zinc finger-based transcription activator.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2549028 SequenceAndFeatures</partinfo>
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<!-- Add more about the biology of this part here -->
 
<!-- Add more about the biology of this part here -->
 
===Biology===
 
===Biology===
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====synthetic promotor operators regulated by artificial zinc finger-based transcription factors====
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=====It works as we designed =====
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JJ Collins et al have reported several synthetic promotor operators which can interact with artificial zinc finger-based transcription factors with high specificity and high orthogonality<ref>A synthetic biology framework for programming eukaryotic transcription functions. Khalil AS, Lu TK, Bashor CJ, ..., Joung JK, Collins JJ. Cell, 2012 Aug;150(3):647-58  PMID: 22863014; DOI: 10.1016/j.cell.2012.05.045</ref>.
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interaction between ZF43.8-VP64 and 8*ZF43.8-minCMV promotor
  
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[[File:ZF2.jpg|none|540px|thumb|JJ Collins et al stated:''sTFs constructed from OPEN-engineered ZFs are orthogonal to one another. sTF43-8 activated noncognate Promoter21-16 due to the fortuitous creation of a sequence that is significantly similar to the binding sequence of 43-8, when the downstream BamHI restriction site is considered.'']]
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====interaction between ZF43.8-VP64 and 8*ZF43.8-minCMV promotor====
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<!-- -->
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=====Synthetic promotor operators regulated by artificial zinc finger-based transcription factors=====
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<span class='h3bb'>Sequence and Features</span>
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Khalil AS et al have reported several synthetic promotor operators which can interact with artificial zinc finger-based transcription factors with high specificity and high orthogonality<ref>A synthetic biology framework for programming eukaryotic transcription functions. Khalil AS, Lu TK, Bashor CJ, ..., Joung JK, Collins JJ. Cell, 2012 Aug;150(3):647-58  PMID: 22863014; DOI: 10.1016/j.cell.2012.05.045</ref>.
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<partinfo>BBa_K2549028 SequenceAndFeatures</partinfo>
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[[File:ZF2.jpg|none|540px|thumb|Khalil AS et al stated:''sTFs constructed from OPEN-engineered ZFs are orthogonal to one another. sTF43-8 activated noncognate Promoter21-16 due to the fortuitous creation of a sequence that is significantly similar to the binding sequence of 43-8, when the downstream BamHI restriction site is considered.'']]
  
  
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K2549028 parameters</partinfo>
 
<partinfo>BBa_K2549028 parameters</partinfo>
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===References===
 
===References===

Revision as of 14:16, 12 October 2018


8*ZF43.8-minCMV

This part is one of the response elements of our amplifier, also executing the combiner function. 8*ZF43.8 binding sites is assembled using two 4*ZF43.8 binding sites (Part:BBa_K2446006) with a biobrick scar between them. Minimal CMV (Part:BBa_K2549049) is a promotor providing very low basal expression and high maximal expression after induction. This part can switch on the expression of gene downstream after induced by our zinc finger-based transcription activator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Biology

It works as we designed

interaction between ZF43.8-VP64 and 8*ZF43.8-minCMV promotor

@@@@

Synthetic promotor operators regulated by artificial zinc finger-based transcription factors

Khalil AS et al have reported several synthetic promotor operators which can interact with artificial zinc finger-based transcription factors with high specificity and high orthogonality[1].

Khalil AS et al stated:sTFs constructed from OPEN-engineered ZFs are orthogonal to one another. sTF43-8 activated noncognate Promoter21-16 due to the fortuitous creation of a sequence that is significantly similar to the binding sequence of 43-8, when the downstream BamHI restriction site is considered.


References

  1. ↑ A synthetic biology framework for programming eukaryotic transcription functions. Khalil AS, Lu TK, Bashor CJ, ..., Joung JK, Collins JJ. Cell, 2012 Aug;150(3):647-58 PMID: 22863014; DOI: 10.1016/j.cell.2012.05.045