Difference between revisions of "Part:BBa K2617006"

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==Characterization==
 
==Characterization==
  
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During the production process, the accumulation of butanol will exert a great inhibitory effect on cell growth. Therefore, we will introduce the GroEL gene and GroES gene from clostridium acetone butanol to make escherichia coli heterologous expression of heat shock protein (a molecular chaperone), which has been shown to increase butanol production [1].To test butanol tolerance, the control strain DH5α, and the GroESL overexpression strain DH5α were grown in the presence of butanol in concentrations up to 1%.  
+
During the production process, the accumulation of butanol will exert a great inhibitory effect on cell growth. Therefore, we will introduce the GroEL gene and GroES gene from clostridium acetone butanol to make escherichia coli heterologous expression of heat shock protein (a molecular chaperone), which has been shown to increase butanol production [1].To test butanol tolerance, the control strain DH5α, and the GroESL overexpression strain DH5α were grown in the presence of 1% butanol. Fig. 1 shows that GroESL have a good effect on butanol tolerance.
  
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[[File:T--UESTC-China--GroESL in DH5α.png|500px|thumb|center|'''Fig. 1''' The measurement of optical density (A600) by periodically taking samples.]]
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[[File:T--UESTC-China--GroESL in DH5α.png|500px|thumb|center|'''Fig. 1''' After adding 0.3ml butanol in 30ml LB medium, the measurement of optical density (A600) deference between DH5α with GroESL and Negative Control in 24 hour.]]
  
 
===References===
 
===References===

Revision as of 12:30, 16 October 2018


GroEL

Promoting folding of misfolded polypeptides and enhancing the tolerance of Escherichia coli to butanol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 880
    Illegal AgeI site found at 1341
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

During the production process, the accumulation of butanol will exert a great inhibitory effect on cell growth. Therefore, we will introduce the GroEL gene and GroES gene from clostridium acetone butanol to make escherichia coli heterologous expression of heat shock protein (a molecular chaperone), which has been shown to increase butanol production [1].To test butanol tolerance, the control strain DH5α, and the GroESL overexpression strain DH5α were grown in the presence of 1% butanol. Fig. 1 shows that GroESL have a good effect on butanol tolerance.

Fig. 1 After adding 0.3ml butanol in 30ml LB medium, the measurement of optical density (A600) deference between DH5α with GroESL and Negative Control in 24 hour.

References

1.Zingaro, K. A., & Papoutsakis, E. T. (2013). GroESL overexpression imparts Escherichia coli tolerance to i-, n-, and 2-butanol, 1, 2, 4-butanetriol and ethanol with complex and unpredictable patterns. Metabolic engineering, 15, 196-205.