Difference between revisions of "Part:BBa K2738104"

 
 
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<partinfo>BBa_K2738104 short</partinfo>
 
<partinfo>BBa_K2738104 short</partinfo>
  
Enterocin is an AMP
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Part: BBa_K2738104 (designed protein lyase cage-enterocin A)
Fusion protein
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Protein lyase cage is designed synthetically to form a multimer
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Description
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We aimed to fuse antimicrobial peptides to self-assembling multimeric proteins giving us star-shape antimicrobial peptides called StarCores. The StarCore described here is a result of the fusion of protein-lyase-cage (self-assembling protein) to Enterocin A (antimicrobial peptide) via golden-gate assembly. The fusion protein expression is regulated by T7-promoter. 
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The protein-lyase-cage is a multimeric, self-assembling protein formed by synthetically designed monomeric subunits. Enterocin A is an antimicrobial peptide, naturally produced by Enterococcus faecium.
 
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===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 00:40, 18 October 2018


Enterocin fused to N-ter designed protein lyase cage monomer

Part: BBa_K2738104 (designed protein lyase cage-enterocin A)

Description We aimed to fuse antimicrobial peptides to self-assembling multimeric proteins giving us star-shape antimicrobial peptides called StarCores. The StarCore described here is a result of the fusion of protein-lyase-cage (self-assembling protein) to Enterocin A (antimicrobial peptide) via golden-gate assembly. The fusion protein expression is regulated by T7-promoter.

The protein-lyase-cage is a multimeric, self-assembling protein formed by synthetically designed monomeric subunits. Enterocin A is an antimicrobial peptide, naturally produced by Enterococcus faecium. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 743
  • 1000
    COMPATIBLE WITH RFC[1000]