Difference between revisions of "Part:BBa K2738104"
Line 3: | Line 3: | ||
<partinfo>BBa_K2738104 short</partinfo> | <partinfo>BBa_K2738104 short</partinfo> | ||
− | + | Part: BBa_K2738104 (designed protein lyase cage-enterocin A) | |
− | + | ||
− | + | ||
+ | Description | ||
+ | We aimed to fuse antimicrobial peptides to self-assembling multimeric proteins giving us star-shape antimicrobial peptides called StarCores. The StarCore described here is a result of the fusion of protein-lyase-cage (self-assembling protein) to Enterocin A (antimicrobial peptide) via golden-gate assembly. The fusion protein expression is regulated by T7-promoter. | ||
+ | |||
+ | The protein-lyase-cage is a multimeric, self-assembling protein formed by synthetically designed monomeric subunits. Enterocin A is an antimicrobial peptide, naturally produced by Enterococcus faecium. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Latest revision as of 00:40, 18 October 2018
Enterocin fused to N-ter designed protein lyase cage monomer
Part: BBa_K2738104 (designed protein lyase cage-enterocin A)
Description We aimed to fuse antimicrobial peptides to self-assembling multimeric proteins giving us star-shape antimicrobial peptides called StarCores. The StarCore described here is a result of the fusion of protein-lyase-cage (self-assembling protein) to Enterocin A (antimicrobial peptide) via golden-gate assembly. The fusion protein expression is regulated by T7-promoter.
The protein-lyase-cage is a multimeric, self-assembling protein formed by synthetically designed monomeric subunits. Enterocin A is an antimicrobial peptide, naturally produced by Enterococcus faecium. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 743
- 1000COMPATIBLE WITH RFC[1000]