Difference between revisions of "Part:BBa K2738006"

 
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2738006 short</partinfo>
 
<partinfo>BBa_K2738006 short</partinfo>
 +
Part: BBa_K2738006 (Ferritin-ovispirin)
 +
Description
 +
We aimed to fuse antimicrobial peptides to self-assembling multimeric proteins giving us star-shape antimicrobial peptides called StarCores. The StarCore described here is a result of the fusion of Ferritin (self-assembling protein) to ovispirin (antimicrobial peptide) via golden-gate assembly. The fusion protein expression is regulated by T7-promoter. 
 +
 +
Ferritin is a universal intracellular protein produced by almost all living organisms, including algae, bacteria, higher plants, and animals. Ferritin is a globular protein complex consisting of 24 protein subunits forming a nanocage. Ovispirin is an alpha-helical antimicrobial peptide derived from derived SMAP29 peptide.It was found to inhibit several antibiotic-resistant bacterial strains.
 +
 +
Using homology modelling, we obtained the structure of ferritin-ovispirin fusion monomer protein. Using homology alignment, we were able to reconstruct the assembled ferritin-ovispirin monomer into the multimeric star-shaped construct. 
 +
<img scr="https://static.igem.org/mediawiki/2018/f/f8/T--Paris_Bettencourt--c6_monomer.gif">
 +
Monomer
 +
 +
<img scr="https://static.igem.org/mediawiki/2018/f/fc/T--Paris_Bettencourt--c6_assemble.gif">
 +
Assembly
 +
  
−
Ovispirin is an AMP
 
−
Ferritin is iron chelating multimeric complex protein
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 00:30, 18 October 2018


Ovispirin fused to N-ter of Ferritin protein Part: BBa_K2738006 (Ferritin-ovispirin) Description We aimed to fuse antimicrobial peptides to self-assembling multimeric proteins giving us star-shape antimicrobial peptides called StarCores. The StarCore described here is a result of the fusion of Ferritin (self-assembling protein) to ovispirin (antimicrobial peptide) via golden-gate assembly. The fusion protein expression is regulated by T7-promoter.

Ferritin is a universal intracellular protein produced by almost all living organisms, including algae, bacteria, higher plants, and animals. Ferritin is a globular protein complex consisting of 24 protein subunits forming a nanocage. Ovispirin is an alpha-helical antimicrobial peptide derived from derived SMAP29 peptide.It was found to inhibit several antibiotic-resistant bacterial strains.

Using homology modelling, we obtained the structure of ferritin-ovispirin fusion monomer protein. Using homology alignment, we were able to reconstruct the assembled ferritin-ovispirin monomer into the multimeric star-shaped construct. <img scr="T--Paris_Bettencourt--c6_monomer.gif"> Monomer

<img scr="T--Paris_Bettencourt--c6_assemble.gif"> Assembly


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]