Difference between revisions of "Part:BBa K2617006"
| Line 14: | Line 14: | ||
==Characterization== | ==Characterization== | ||
| − | [[File:T--UESTC-China--GroESL in DH5α.png|500px|thumb|center|'''Fig. 1''']] | + | During the production process, the accumulation of butanol will exert a great inhibitory effect on cell growth. Therefore, we will introduce the GroEL gene and GroES gene from clostridium acetone butanol to make escherichia coli heterologous expression of heat shock protein -- a molecular chaperone, which has been shown to increase butanol production [1].To test butanol tolerance, the control strain DH5α, and the GroESL overexpression strain DH5α were grown in the presence of butanol in concentrations up to 1%. |
| + | |||
| + | [[File:T--UESTC-China--GroESL in DH5α.png|500px|thumb|center|'''Fig. 1''' The measurement of optical density (A600) by periodically taking samples.]] | ||
Revision as of 12:35, 10 October 2018
GroEL
Promoting folding of misfolded polypeptides and enhancing the tolerance of Escherichia coli to butanol.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 880
Illegal AgeI site found at 1341 - 1000COMPATIBLE WITH RFC[1000]
Characterization
During the production process, the accumulation of butanol will exert a great inhibitory effect on cell growth. Therefore, we will introduce the GroEL gene and GroES gene from clostridium acetone butanol to make escherichia coli heterologous expression of heat shock protein -- a molecular chaperone, which has been shown to increase butanol production [1].To test butanol tolerance, the control strain DH5α, and the GroESL overexpression strain DH5α were grown in the presence of butanol in concentrations up to 1%.
