Difference between revisions of "Part:BBa K2541404:Design"

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===Source===
 
===Source===
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sfGFP(BbsI free) has two mutations from superfolder GFP(BBa_I746916). And other sequences are derived from Registry of Standard Biological Parts.
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E
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===References===
 
===References===

Revision as of 11:00, 10 October 2018


sfGFP(BbsI free) measurement device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 74


Design Notes

D


Source

sfGFP(BbsI free) has two mutations from superfolder GFP(BBa_I746916). And other sequences are derived from Registry of Standard Biological Parts.

References

[1]Southward CM, Surette MG. 2002. The dynamic microbe: green fluorescent protein brings bacteria to light. Mol. Microbiol. 45:1191–1196.

[2]Cormack BP, Valdivia RH, Falkow S. 1996. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173:33–38.

[3]Pédelacq J-D, Cabantous S, Tran T, Terwilliger TC, Waldo GS. 2006. Engineering and characterization of a superfolder green fluorescent protein. Nat. Biotechnol. 24:79 –88.