Difference between revisions of "Part:BBa K2617000"

Revision as of 16:05, 10 October 2018

Xyn10D-Fae1A：Bifunctional xylanase/ferulic acid esterase

Xyn1D-fae1A cuts off the crosslinking between polysaccharides and lignin in cell wall and degrade xylose hemicellulose. This part is responsible for pretreatment of straw.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 534
Illegal AgeI site found at 381
Illegal AgeI site found at 628
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1236

Characterization

Molecular weight

This halohydrin dehalogenase gene codes for a protein of 726 amino acids with a molecular mass of 82.3 kDa.

Enzyme activity

Fig. 1 Standard Curve of Xylose. Data were measured in phosphate buffer solution at pH 6.0 and 40℃.

Fig. 2 Xylanase Activity on Xylan. Data were measured inphosphate buffer solution at pH 6.0 and 40℃.

Fig. 3 Enzyme Activity of Ferulic acid esterase. Data were measured in buffer at pH 6.0 and 40℃.

@@ Line 21: / Line 21: @@
-[[File:T--UESTC-China-standard curve of xylose .png|500px|thumb|center|'''Fig. 1''' Standard Curve of Xylose. Data were measured in  buffer at pH 6.0 and 40℃.]]
+[[File:T--UESTC-China-standard curve of xylose .png|500px|thumb|center|'''Fig. 1''' Standard Curve of Xylose. Data were measured in phosphate buffer solution at pH 6.0 and 40℃.]]
-[[File:T--UESTC-China-xylanase activity.png|500px|thumb|center|'''Fig. 2''' Xylanase Activity on Xylan. Data were measured in  buffer at pH 6.0 and 40℃.]]
+[[File:T--UESTC-China-xylanase activity.png|500px|thumb|center|'''Fig. 2''' Xylanase Activity on Xylan. Data were measured inphosphate buffer solution at pH 6.0 and 40℃.]]
 [[File:T--UESTC-China--Enzyme Activity of Ferulic acid esterase.png|500px|thumb|center|'''Fig. 3''' Enzyme Activity of Ferulic acid esterase. Data were measured in  buffer at pH 6.0 and 40℃.]]