Difference between revisions of "Part:BBa K2610035"
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As was demonstrated in Figure 1 pSoxS-GFP-GFP-pSoxS-GFP-GFP responds to nalidixic acid in a dose-dependent response curve. | As was demonstrated in Figure 1 pSoxS-GFP-GFP-pSoxS-GFP-GFP responds to nalidixic acid in a dose-dependent response curve. | ||
| − | [[File:T--Leiden-- | + | [[File:T--Leiden--amplification4.png|750px]] |
<span style="font-size:1em"><b>Figure 1.</b> Dose-response curve as a result of treatment with nalidixic acid. </span> | <span style="font-size:1em"><b>Figure 1.</b> Dose-response curve as a result of treatment with nalidixic acid. </span> | ||
Revision as of 12:01, 11 October 2018
pSoxS-GFP-GFP-pSoxS-GFP-GFP
This composite part features twice the regulatory part promoter SoxS (BBa_K2610030) and two times the fluorescent protein GFP (BBa_E0040). It can be used to visualize upregulation of SoxS as a result of superoxide stress.
Regulatory protein SoxS is involved in the superoxide pathway in Escherichia coli. It acts a superactivator of downstream stress genes.
Usage and Biology
We, iGEM Leiden 2018, have designed this composite part as part of our project Fifty Shades of Stress. This reporter part allowed us to detect stress-induced changes in SoxS transcription. We have created this part in order to amplify the fluorescent signal measured after pSoxS-GFP activation.
As was demonstrated in Figure 1 pSoxS-GFP-GFP-pSoxS-GFP-GFP responds to nalidixic acid in a dose-dependent response curve.
Figure 1. Dose-response curve as a result of treatment with nalidixic acid.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 947
Illegal BsaI.rc site found at 1673
Illegal BsaI.rc site found at 2704
Illegal BsaI.rc site found at 3430