Difference between revisions of "Part:BBa K2610032"

Revision as of 12:47, 10 October 2018

pSoxS-mCherry

This composite part features the regulatory part promoter SoxS (BBa_K2610030) and the fluorescent protein mCherry (BBa_J06504). It can be used to visualize upregulation of SoxS as a result of superoxide stress.

Regulatory protein SoxS is involved in the superoxide pathway in Escherichia coli. It acts a superactivator of downstream stress genes. We have coupled this promoter to mCherry, a second generation monomeric fluorescent protein.

Usage and Biology

We, iGEM Leiden 2018, have used confocal microscopy in order to visualize the E.coli stress response to nalidixic acid. We incubated our transformed DH5a cells with 100 ug/mL nalidixic acid for 3 hours, after which they were analysed with the confocal (Figure 1). As can be observed, treating the cells with nalidixic acid resulted in increased fluorescence.

Figure 1. pSoxS-mCherry transformed cells untreated (A and B) and treated with 100 ug/mL nalidixic acid.

Sequence and Features