Difference between revisions of "Part:BBa K2610030"

Revision as of 23:29, 17 October 2018

pSoxS

Regulatory protein SoxS is involved in the oxidative stress signaling pathway in Escherichia coli. Intracellular superoxide-generating compounds cause the SoxR to activate transcription of SoxS, which then triggers a set of defense and repair genes that form the oxidative response system.

Usage and Biology

iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. For this, e have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry. We fused this promotor to green fluorescent protein (see part BBa_K2610031) and stressed transformed DH5a cells for several hours.

Figure 1. Heatmap with stress-activated promoters

As can be observed in Figure 1 the SoxS promoter is specifically activated by nalidixic acid and hydrogen peroxide, which both cause oxidative stress.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2610030 short</partinfo>
-Regulatory protein SoxS is involved in the superoxide pathway in Escherichia coli. It acts a superactivator of downstream stress genes.
+Regulatory protein SoxS is involved in the oxidative stress signaling pathway in Escherichia coli. Intracellular superoxide-generating compounds cause the SoxR to activate transcription of SoxS, which then triggers a set of defense and repair genes that form the oxidative response system.
 ===Usage and Biology===
-iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry.
+iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. For this, e have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry.
-We fused promoters to green fluorescent protein and stressed transformed DH5a cells for several hours.
+We fused this promotor to green fluorescent protein (see part [https://parts.igem.org/Part:BBa_K2610031 BBa_K2610031]) and stressed transformed DH5a cells for several hours.
 [[File:T--Leiden--heatmap.png|750px]]
@@ Line 14: / Line 15: @@
 <span style="font-size:1em"><b>Figure 1.</b> Heatmap with stress-activated promoters </span>
-As can be observed in Figure 1 the SoxS promoter is specifically activated by nalidixic acid and hydrogen peroxide.
+As can be observed in Figure 1 the SoxS promoter is specifically activated by nalidixic acid and hydrogen peroxide, which both cause oxidative stress.
 <!-- Add more about the biology of this part here