Difference between revisions of "Part:BBa I50042"
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<partinfo>BBa_I50042 short</partinfo> | <partinfo>BBa_I50042 short</partinfo> | ||
| − | [[Part:BBa_I50042|BBa_I50042]] is a pSC101 origin of replication. [[Part:BBa_I50042|BBa_I50042]] is the replication origin found in the pSB4* series of BioBrick vectors. | + | [[Part:BBa_I50042|BBa_I50042]] is a pSC101 origin of replication. [[Part:BBa_I50042|BBa_I50042]] is the replication origin found in the pSB4*5 series of BioBrick vectors. |
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 23:23, 25 September 2012
pSC101 origin of replication
BBa_I50042 is a pSC101 origin of replication. BBa_I50042 is the replication origin found in the pSB4*5 series of BioBrick vectors.
Usage and Biology
- BBa_I50042 yields vectors with an expected copy number of ~5 per cell ([http://openwetware.org/wiki/Molecular_Cloning Molecular Cloning]).
References
Engineering BioBrick vectors from BioBrick parts
Journal of Biological Engineering, 2008 Apr 14;2:5
Reshma Shetty, Drew Endy, Tom Knight
[http://www.jbioleng.org/content/2/1/5 URL]
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=169656107 GenBank EU496096]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]