Difference between revisions of "Part:BBa K2787013"
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The 16S rRNA BBa_K2787040 under constitutive promoter BBa_J23100(BBa_K2787008) and pCons-oRBSB8-GFP(BBa_K2787001) are linked into one plasmid to quantify the orthogonality of the TJU and B8 ribosome system. | The 16S rRNA BBa_K2787040 under constitutive promoter BBa_J23100(BBa_K2787008) and pCons-oRBSB8-GFP(BBa_K2787001) are linked into one plasmid to quantify the orthogonality of the TJU and B8 ribosome system. | ||
− | <!-- Add more about the biology of this part | + | <!-- Add more about the biology of this part her |
− | + | ||
+ | ===Usage and Biology=== | ||
+ | [[File:ShanghaiTech2018-b8 rbs.png|700px|center]] | ||
+ | [[File:ShanghaiTech-TJU_16S.png|700px|center]] | ||
+ | [[File:A2-B8-C9-Orthogonality.png|700px|center]] | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Revision as of 22:48, 17 October 2018
GFP under B8 orthogonal RBS with orthogonal 16S rRNA BBa_K2787041
The 16S rRNA BBa_K2787040 under constitutive promoter BBa_J23100(BBa_K2787008) and pCons-oRBSB8-GFP(BBa_K2787001) are linked into one plasmid to quantify the orthogonality of the TJU and B8 ribosome system.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 798
Illegal NheI site found at 821 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1661
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1675
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 707