Difference between revisions of "Part:BBa K2668010:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| − | The part BBa_K2668010 comes from the <a href="http://2018.igem.org/Team:Toulouse-INSA-UPS/Design">Cerberus Project</a>. It was design for binding cellulose to different types of compounds at the same time thanks to its two very unlike linkers. The streptavidin linker can bind biotin and biotinylated compounds while the UnAA-containing linker will click molecules that bear an alkyne function. | + | The part BBa_K2668010 comes from the <a href="http://2018.igem.org/Team:Toulouse-INSA-UPS/Design">iGEM Team Toulouse-INSA-UPS Cerberus Project</a>. It was design for binding cellulose to different types of compounds at the same time thanks to its two very unlike linkers. The streptavidin linker can bind biotin and biotinylated compounds while the UnAA-containing linker will click molecules that bear an alkyne function. |
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Revision as of 19:11, 10 October 2018
Cerberus : A Molecular Binding Plateform (mSA2-CBM3a-AzF)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 358
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part BBa_K2668010 comes from the iGEM Team Toulouse-INSA-UPS Cerberus Project. It was design for binding cellulose to different types of compounds at the same time thanks to its two very unlike linkers. The streptavidin linker can bind biotin and biotinylated compounds while the UnAA-containing linker will click molecules that bear an alkyne function.
Source
The Part was obtained by IDT gBlock synthesis. The CBM3a domain is a corrected version of the Part:BBa_K1321014, RFC10 compatible . The monomeric streptavidin is the Part:BBa_K1896000.