Difference between revisions of "Part:BBa K2614000:Design"
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===Source=== | ===Source=== | ||
− | As template we used a synthetic oligonucleotide. | + | As template we used a short, synthetic, non toxic, non pathogene oligonucleotide. |
===References=== | ===References=== |
Revision as of 22:00, 15 October 2018
template as positive control for a qPCR that detects Plasmodium
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We amplifiy the template by a PCR and digest it with EcoRI-HF an PstI-HF. Subsequently we digest the pSB1C3 plasmid with EcoRI-HF and PstI-HF and ligate the digested Plasmid with our digested template.
Source
As template we used a short, synthetic, non toxic, non pathogene oligonucleotide.