Difference between revisions of "Part:BBa K2669003"
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<partinfo>BBa_K2669003 short</partinfo> | <partinfo>BBa_K2669003 short</partinfo> | ||
| − | Composite part containing codon optimized original native AmilCP [[BBa_K2669002]], RBS [[BBa_J34801]], constitutive promotor [[BBa_J23119]] and a double terminator [[BBa_B0014]]. The part contains a blue chromoprotein which naturally exhibits strong color when expressed that can be observed with naked eye in both LB and agar culture. | + | Composite part containing codon optimized original native AmilCP [[Part:BBa_K2669002]], RBS [[Part:BBa_J34801]], constitutive promotor [[Part:BBa_J23119]] and a double terminator [[Part:BBa_B0014]]. The part contains a blue chromoprotein which naturally exhibits strong color when expressed that can be observed with naked eye in both LB and agar culture. |
The chromoprotein was first codon optimized by Austin Texas 2017 iGEM team. By performing codon optimization they hypothesized that the seemingly unstable original native amilCP from 2011 would become more stable. The Uppsala 2017 team have codon optimized the original native AmilCP further in order to get better proof of improved stability of the protein. | The chromoprotein was first codon optimized by Austin Texas 2017 iGEM team. By performing codon optimization they hypothesized that the seemingly unstable original native amilCP from 2011 would become more stable. The Uppsala 2017 team have codon optimized the original native AmilCP further in order to get better proof of improved stability of the protein. | ||
Revision as of 17:54, 9 October 2018
Strong constitutive promotor + RBS + Optimized Amil CP (BBa_K2669002) + double terminator
Composite part containing codon optimized original native AmilCP Part:BBa_K2669002, RBS Part:BBa_J34801, constitutive promotor Part:BBa_J23119 and a double terminator Part:BBa_B0014. The part contains a blue chromoprotein which naturally exhibits strong color when expressed that can be observed with naked eye in both LB and agar culture.
The chromoprotein was first codon optimized by Austin Texas 2017 iGEM team. By performing codon optimization they hypothesized that the seemingly unstable original native amilCP from 2011 would become more stable. The Uppsala 2017 team have codon optimized the original native AmilCP further in order to get better proof of improved stability of the protein.
Part can be used as a reporter and is expressed constitutively.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]