Difference between revisions of "Part:BBa K2559001"

Revision as of 15:55, 14 October 2018

Bacterial cellulose synthase H (BcsH)

The BBa_K2559001 is coding a cellulose complement protein A (ccpA) . https://www.sciencedirect.com/science/article/pii/S0966842X15001201#tbl0005

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 746
Illegal AgeI site found at 907
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 351

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 <partinfo>BBa_K2559001 short</partinfo>
-BcsH expresses cellulose complement protein A.It affects the expression levels of BcsB and BcsC, interacts with BcsD, and appears to assist the arrangement of glucan chains into crystalline ribbons.
+The BBa_K2559001 is coding a cellulose complement protein A (ccpA) .
 https://www.sciencedirect.com/science/article/pii/S0966842X15001201#tbl0005
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
-We introduced the Acetobacter xylinum bacterial cellulose synthase gene (including BcsZ, H, A, B, C, D, LX) into the model organism Synechocystis and successfully expressed it,but we have not explored its individual function. The part is facilitate the study of its individual functions for other teams
+It is encoded exclu-sively in the Komagataeibacter/Gluconacetobacter lineage and is required for the BCS activity in K. xylinus and Komagataeibacter hansenii  It has been shown to interact with BcsD, and appears to assist the arrangement of glucan chains into crystalline ribbons. in the periplasm. This unique organization might account for the extremely high activity of the K. xylinus BCS.
+[[File:Scau-china-2018-6.png|800px|thumb|center| Figure 1 The double digestion of biobrick by EcoRI and pstI]]
+Transgenic cyanobacteria with BcsZH-ABCD-BglX genes and the cellulose measurement
+Cyanobacteria glucose content measurement (3 repeats). The same of quality of transferred cyanobacteria with BcsZH-ABCD-BglX gene and wildtype are treated with lysozyme to break out the cell. The glucose represents the content of cellulose. The addition of cellulase can digest the cellulose to glucose then the bacterial cellulose can measured. The distinct differences between the red column and blue column indicate the high expression intensity of bacteria cellulose. Wilcoxon test indicates our data is reliable.
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 <span class='h3bb'>Sequence and Features</span>