Difference between revisions of "Part:BBa K2703001"
 
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<h3> 1- Biological background </h3>
 
<h3> 1- Biological background </h3>
 +
otsA is the first of the two gene in the pathway of trehalose in Escherischia coli.
 +
It catalyzes the transfer of glucose from UDP-alpha-D-glucose (UDP-Glc) to D-glucose 6-phosphate (Glc-6-P) to form trehalose-6-phosphate (Uniprot).
  
 
<h3> 2- Usage in iGEM projects </h3>
 
<h3> 2- Usage in iGEM projects </h3>
−
otsA is the first of the two gene in the pathway of trehalose in Escherischia coli.
+
−
It catalyzes the transfer of glucose from UDP-alpha-D-glucose (UDP-Glc) to D-glucose 6-phosphate (Glc-6-P) to form trehalose-6-phosphate (Uniprot).
+
  
 
=='''Sequencing'''==
 
=='''Sequencing'''==

Latest revision as of 16:34, 9 October 2018

otsA

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 361
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 361
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 485
    Illegal BglII site found at 1040
    Illegal BamHI site found at 383
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 361
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 361
    Illegal NgoMIV site found at 61
    Illegal NgoMIV site found at 103
    Illegal NgoMIV site found at 712
    Illegal NgoMIV site found at 952
    Illegal NgoMIV site found at 1165
  • 1000
    COMPATIBLE WITH RFC[1000]

Introduction

1- Biological background

otsA is the first of the two gene in the pathway of trehalose in Escherischia coli. It catalyzes the transfer of glucose from UDP-alpha-D-glucose (UDP-Glc) to D-glucose 6-phosphate (Glc-6-P) to form trehalose-6-phosphate (Uniprot).

2- Usage in iGEM projects


Sequencing

Characterization

References

  1. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii Pierre Crozet, Francisco J. Navarro, Felix Willmund, Payam Mehrshahi, Kamil Bakowski, Kyle J. Lauersen, Maria-Esther Pérez-Pérez, Pascaline Auroy, Aleix Gorchs Rovira, Susana Sauret-Gueto, Justus Niemeyer, Benjamin Spaniol, Jasmine Theis, Raphael Trösch, Lisa-Desiree Westrich, Konstantinos Vavitsas, Thomas Baier, Wolfgang Hübner, Felix de Carpentier, Mathieu Cassarini, Antoine Danon, Julien Henri, Christophe H. Marchand, Marcello de Mia, Kevin Sarkissian, David C. Baulcombe, Gilles Peltier, José-Luis Crespo, Olaf Kruse, Poul-Erik Jensen, Michael Schroda, Alison G. Smith, and Stéphane D. Lemaire ACS Synthetic Biology 2018 7 (9), 2074-2086

    2. DOI: 10.1021/acssynbio.8b00251

  2. Weber, E., Engler, C., Gruetzner, R., Werner, S. & Marillonnet, S. A modular cloning system for standardized assembly of multigene constructs. PLoS One 6, (2011).